h-index: 7     i10-index: 6

Document Type : Short Communication


1 Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

2 Bahaman Faculty of Medical Sciences, Bahaman, Iran


Introduction: In beta-thalassemia, an imbalance in the production of beta subunits of hemoglobin leads to the oxidation and deposition of excess alpha-globin chains at the cell membrane, resulting in the hemolysis of erythrocytes and a disorder of erythropoiesis. Antioxidants, such as curcumin, may promote this progression. This study aims to investigate the antioxidant effect of curcumin on hemolysate samples from patients with beta-thalassemia.
Materials and methods: Pure curcumin was extracted and purified for use in studying its effect on the visual light absorbance of hemoglobin in hemolysate samples from beta-thalassemia patients compared to control samples. Changes in light absorbance at 540 and 700 nm wavelengths during exposure to curcumin were analyzed to examine the shift from oxyhemoglobin to deoxyhemoglobin.
Results: Curcumin was found to dissolve rapidly and to a high degree in ethanol at 1 mg/ml, but did not dissolve in distilled water at the same concentration. The curcumin addition to the hemolysate sample of a patient with beta-thalassemia resulted in a decrease in the light absorbance of the sample at 540 nm wavelength, with minimal changes observed in the control sample.
Conclusion: Curcumin deoxygenated the hemolysate samples from both the patient and control, causing hemoglobin precipitation to occur slowly. The study suggests a greater potential role for curcumin in deoxygenating hemoglobin in the hemolysate samples of beta-thalassemia patients compared to those of the normal control.


Main Subjects


©2024 The author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit: http://creativecommons.org/licenses/by/4.0/


Sami Publishing Company remains neutral concerning jurisdictional claims in published maps and institutional affiliations.


Sami Publishing Company


[1] Galanello R., Origa R., Beta-thalassemia, Orphanet Journal of Rare Diseases,2010,  5:1 [Crossref], [Google Scholar], [Publisher]‎
[2] Madan U., Bhasin H., Dewan P., Madan J., Bhasin H., Improving ineffective erythropoiesis in thalassemia: a hope on the horizon, Cureus, 2021, 13 [Crossref], [Google Scholar], [Publisher]‎
[3] Che Yaacob N.S., Islam M.A., Alsaleh H., Ibrahim I.K., Hassan R., Alpha-hemoglobin-stabilizing protein (AHSP): A modulatory factor in β-thalassemia, International Journal of Hematology, 2020, 111:352 [Crossref], [Google Scholar], [Publisher]‎
[4] dos Santos C.O., Costa F.F., AHSP and β-thalassemia: A possible genetic modifier. Hematology, 2005, 10:157 [Crossref], [Google Scholar], [Publisher]‎
[5] National Center for Biotechnology Information. PubChem Compound Summary for CID 969516, Curcumin. Retrieved, 2022 [Publisher]
[6] Moinipour N., Barati M., Sahebkar A., Iranshahy M., Shakeri A., Protective effects of curcumin against iron-induced toxicity, Current Pharmaceutical Biotechnology, 2022, 23:1020 [Crossref], [Google Scholar], [Publisher]‎
[7] Jiang  T., Ghosh R., Charcosset C., Extraction, purification and applications of curcumin from plant materials-A comprehensive review,  Trends in Food Science & Technology, 2021, 11:419 [Crossref], [Google Scholar], [Publisher]‎
[8] Wu  F.Y., Sun M.Z., Xiang Y.L., Wu Y.M. Tong  D.Q., Curcumin as a colorimetric and fluorescent chemosensor for selective recognition of fluoride ion. Journal of Luminescence, 2010, 130:304 [Crossref], [Google Scholar], [Publisher]‎
[9] Subhan M.A., Alam K., Rahaman M.S., Rahman  M.A. Awal R., Synthesis and characterization of metal complexes containing curcumin (C21H20O6) and study of their anti-microbial activities and DNA-binding properties. Journal of Scientometric Research, 2014, 6:97 [Crossref], [Google Scholar], [Publisher]‎
[10] Jenie R.P., Nasiba U., Rahayu I., Nurdin N.M., Husein I., Alatas H., December. Review on wavelength for non-invasive blood hemoglobin level measurement optical device. In AIP Conference Proceedings ,  AIP Publishing, 2019,  2194:1 [Crossref], [Google Scholar], [Publisher]‎
[11] Robles F.E., Chowdhury  S., Wax A., Assessing hemoglobin concentration using spectroscopic optical coherence tomography for feasibility of tissue diagnostics. Biomedical Optics Express, 2010, 1:310 [Crossref], [Google Scholar], [Publisher]‎
[12] Yousefi R., Mokarmian S., Jamshidi A., Efficacy of beta-secretase-1 enzyme inhibitors in Alzheimer's disease, Journal of Advanced Pharmacy Research, 2023, 7:243 [Crossref], [Google Scholar], [Publisher]‎
[13] Yousefi R., Mokarmian R., Jamshidi A., Molecular docking of pepstatin (A) and compounds with structural similarity to the molecular model of human BACE-1 enzyme, Journal of Advanced Pharmacy Research, 2023, 7:181 [Crossref], [Google Scholar], [Publisher]‎
[14] Yousefi R., Binding of curcumin near the GBT440 binding site at the alpha cleft in the sickle cell hemoglobin model [Pdb ID: 1NEJ],  Journal of advanced Biomedical and Pharmaceutical Sciences, 2024, 70 [Crossref], [Google Scholar], [Publisher]‎
[15] Yousefi R., The Relationship between the average annual temperature of different countries and the rate of infection and mortality due to covid-19 infection. Eurasian Journal of Science and Technology, 2024, 4:264 [Crossref], [Publisher]‎