h-index: 7     i10-index: 6

Document Type : Original Research Article


Department of Biotechnology, School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 431606, India


Since ancient times, Ajwain is one of the popular ayurvedic medicines in the treatment of indigestion, dyspepsia, and gastric disorders. Phytochemical constituents of Ajwain, are day by day getting more attention as medicine because of their efficiency and safer, and also comes with very less side effects. The natural compounds obtained from this ethno-botanical plants are received much more attention in last decades.
The present investigation is focus on the phytochemical analysis of Ajwain seeds which is commonly used as one of the important ingredients of Indian mouth fresheners. The crude extract of Ajwain seeds were obtained through soxhletion in chloroform and water. The presence of secondary metabolites in the Ajwain seeds was done by qualitative and quantitative analysis. The qualitative analysis of crude aqueous extract of Ajwain seeds shown the presence of Cardiac glycosides, as one of the very important cardiac drug used in the heart treatments.


Main Subjects


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[1] Anwar S., Ahmed N., Habibatni S., Abusamra Y., in Essential Oils in Food Preservation, Flavor and Safety     181 (Elsevier, 2016 [Crossref], [Google Scholar], [Publisher]
[2] Goyal S., Chaturvedi, V., Dhingra, G., Tanwar, S., Sharma, K.,Singh, S., Trachyspermumammi: A review on traditional and modern pharmacological aspects, Biological Sciences, 2022, 2:324 [Crossref], [Google Scholar], [Publisher]
[3] Boskabady M.H., Alitaneh S., Alavinezhad A., Carumcopticum L.: a herbal medicine with various pharmacological effects, BioMed research international, 2014, 2014 [Crossref], [Google Scholar], [Publisher]
[4] RBairwa R., Sodha R., Rajawat B., Trachyspermumammi, Pharmacognosy Reviews, 2012, 6:56 [Crossref], [Google Scholar], [Publisher]
[5] Zarshenas M.M., Moein M., Samani S.M., Petramfar P., An overview on ajwain (Trachyspermumammi) pharmacological effects; modern and traditional, Journal of natural Remedies, 2014, 98 [Crossref], [Google Scholar], [Publisher]
[6] Chauhan B., Kumar G., Ali M., A review on phytochemical constituents and activities of Trachyspermumammi (L.) Sprague fruits, AJPTR, 2012, 2:329 [Google Scholar]
[7] Ranjan B., Sodha R., Rajawat B., Trachyspermumammi, Pharmacognosy Reviews, 2012, 6:56 [Crossref], [Google Scholar], [Publisher]
[8] Lim T.K., Edible medicinal and non-medicinal plants (Vol. 1, pp. 656-687). Dordrecht, The Netherlands: Springer. 2012. [Google Scholar], [Publisher]
[9] Jeet K., Devi N., Thakur N., Tomar S., Shalta L., Thakur R., Trachyspermum ammi: A comprehensive review, International Research Journal of Pharmacy, 2012, 5:138 [Google Scholar]
[10] Mostafavi H., Pezhhanfar S., Qualitative phytochemical analysis of ajwain (Trachyspermum ammi) from north-west Iran. International Research Journal of Pharmacy, 2015, 6:610 [Google Scholar]
[11]Ekor M., The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety, Frontiers in Pharmacology, 2014, 4:177 [Crossref], [Google Scholar], [Publisher]
[12] Khan M.S.A., Ahmad I., in New look to phytomedicine     3 (Elsevier, 2019 [Crossref], [Google Scholar], [Publisher]
[13] Zhang X., Organization, W.H., Traditional medicine strategy 2002 2005, 2002 [Google Scholar], [Publisher]
[14]Igoli J., Igwue I., Igoli N., Traditional medicinal practices among the Igede people of Nigeria, Journal of herbs, spices & medicinal plants, 2004, 10:1 [Crossref], [Google Scholar], [Publisher]
[15] Begum M., Sharma B.P., Sharma H.K., Ethano-botanical, Phytochemical, and Pharmacological Scence of Trachyspermum ammi (Ajwain): A Systematic Review IJPBSTM, 2021, 12:5690 [PDF]
[16] Kumar N., Khurana S., Phytochemicals and bioactive potential of Trachyspermum ammi L. The Pharma Letter, 2018, 10:48 [PDF], [Google Scholar]
[17]Shraim A.M., Ahmed T.A., Rahman M.M., Hijji Y.M., Determination of total flavonoid content by aluminum chloride assay: A critical evaluation, Lwt, 2021, 150:111932 [Crossref], [Google Scholar], [Publisher]
[18] Noreen H., Semmar N., Farman M., McCullagh J.S., Measurement of total phenolic content and antioxidant activity of aerial parts of medicinal plant Coronopusdidymus, Asian Pacific journal of tropical medicine, 2017, 10:792 [Crossref], [Google Scholar], [Publisher]
[19]Peri C., Pompei C., Estimation of different phenolic groups in vegetable extracts, Phytochemistry, 1971, 10:2187 [Crossref], [Google Scholar], [Publisher]
[20] Singh R., Verma P.K., Singh G., Total phenolic, flavonoids and tannin contents in different extracts of Artemisia absinthium, Journal of Complementary Medicine Research, 1970, 1:101 [Crossref], [Google Scholar], [Publisher]
[21]Baliyan S., Mukherjee R., Priyadarshini A., Vibhuti A., Gupta A., Pandey R.P., Chang C.M., Determination of antioxidants by DPPH radical scavenging activity and quantitative phytochemical analysis of Ficusreligiosa, Molecules, 2022, 27:1326 [Crossref], [Google Scholar], [Publisher]
[22]Oluwole O., Fernando W.B., Lumanlan J., Ademuyiwa O., Jayasena V., Role of phenolic acid, tannins, stilbenes, lignans and flavonoids in human health–A review, International Journal of Food Science & Technology, 2022, 57:6326 [Crossref], [Google Scholar], [Publisher]
[23] Osman M.H., Farrag E., Selim M., Osman M.S., Hasanine A., Selim A., Cardiac glycosides use and the risk and mortality of cancer; systematic review and meta-analysis of observational studies, PloS one, 2017, 12:e0178611 [Crossref], [Google Scholar], [Publisher]