Comparison of Exons 2 and 3 of DIRAS3 Gene in Mastectomies and Lumpectomies Women

Kalvanagh, Parmiss Adyani; Kalvanagh, Yousef Adyani

doi:10.48309/ejst.2023.392686.1080

Document Type : Original Research Article

Authors

¹ Postgraduate Student, Experimental Sciences, Tabriz, Iran

² Breast Surgery Fellowship, Tabriz University of Medical Sciences, Tabriz, Iran

https://doi.org/10.48309/ejst.2023.392686.1080

Abstract

Introduction: According to researchers' research on the relationship between breast cancer and diabetes in the presence of other genes such as INSR, the aim of this study was comparing exons 2 and 3 of the DIRAS3 gene in mastectomies and lumpectomies women.

Procedure: DNA extraction was done using a kit (PZP Molecular IVD Company) and stored in a micro tube at -20C. The said kit contained four different buffers named Wash buffer (W), Binding buffer (B), Elution buffer (E) and Removal buffer (R) along with proteinase k powder.

Results: The polymorphism found in intron 2 with rs6682360 at position c.332-3 T>C or g.831 T>C of a cancer patient sample, that is, 3 nucleotides upstream of the 332nd nucleotide in intron 2 or the 831st nucleotide in intron 2 which is T open to C open. Since the number of measured samples was small, no significant relationship was found between the development of breast cancer and diabetes and the presence of this polymorphism.

Conclusion: The results of this research showed that among the available polymorphisms, only the 5'UTR c.331 G>A is related to breast cancer in mastectomies and lumpectomies women.

Keywords

Main Subjects

Biotechnology

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Eurasian Journal of Science and Technology

Comparison of Exons 2 and 3 of DIRAS3 Gene in Mastectomies and Lumpectomies Women

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Volume 3, Issue 3
July 2023
Pages 147-157

Comparison of Exons 2 and 3 of DIRAS3 Gene in Mastectomies and Lumpectomies Women

References

References

Volume 3, Issue 3July 2023Pages 147-157

Volume 3, Issue 3
July 2023
Pages 147-157