Basic Medicine
Roohallah Yousefi
Abstract
Introduction: In beta-thalassemia, an imbalance in the production of beta subunits of hemoglobin leads to the oxidation and deposition of excess alpha-globin chains at the cell membrane, resulting in the hemolysis of erythrocytes and a disorder of erythropoiesis. Antioxidants, such as curcumin, may promote ...
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Introduction: In beta-thalassemia, an imbalance in the production of beta subunits of hemoglobin leads to the oxidation and deposition of excess alpha-globin chains at the cell membrane, resulting in the hemolysis of erythrocytes and a disorder of erythropoiesis. Antioxidants, such as curcumin, may promote this progression. This study aims to investigate the antioxidant effect of curcumin on hemolysate samples from patients with beta-thalassemia.Materials and methods: Pure curcumin was extracted and purified for use in studying its effect on the visual light absorbance of hemoglobin in hemolysate samples from beta-thalassemia patients compared to control samples. Changes in light absorbance at 540 and 700 nm wavelengths during exposure to curcumin were analyzed to examine the shift from oxyhemoglobin to deoxyhemoglobin.Results: Curcumin was found to dissolve rapidly and to a high degree in ethanol at 1 mg/ml, but did not dissolve in distilled water at the same concentration. The curcumin addition to the hemolysate sample of a patient with beta-thalassemia resulted in a decrease in the light absorbance of the sample at 540 nm wavelength, with minimal changes observed in the control sample.Conclusion: Curcumin deoxygenated the hemolysate samples from both the patient and control, causing hemoglobin precipitation to occur slowly. The study suggests a greater potential role for curcumin in deoxygenating hemoglobin in the hemolysate samples of beta-thalassemia patients compared to those of the normal control.
Basic Medicine
Roohallah Yousefi
Abstract
Introduction: Voxelotor, also known as GBT 440, binds to the alpha cleft in HbS tetramers and promotes the stability of the relaxed or oxygenated state of HbS. This process hinders the conformational change of the HbS tetramers into the deoxygenated state. Voxelotor prevents interactions between HbS ...
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Introduction: Voxelotor, also known as GBT 440, binds to the alpha cleft in HbS tetramers and promotes the stability of the relaxed or oxygenated state of HbS. This process hinders the conformational change of the HbS tetramers into the deoxygenated state. Voxelotor prevents interactions between HbS tetramers in the deoxygenated state, ultimately inhibiting the polymerization of HbS tetramers and resulting in significant clinical improvements, particularly in raising hemoglobin levels in patients. In this study, we have explored the use of herbal compound models, such as rosmarinic acid and compounds with similar structures that exhibit high binding affinity to Voxelotor's hemoglobin binding site.Materials and methods: The molecular model of hemoglobin (PDB: 5E83) was initially obtained from the RCSB PDB database. In addition, we collected 453 ligand models with structural similarity to rosmarinic acid from the PubChem database. To prepare these models for molecular docking, we utilized the Molegro Virtual Docker tool. Subsequently, we used the SwissADME web tool to predict the physicochemical properties and pharmacokinetics of these compounds. Results: We investigated the affinity and binding site of 453 compounds similar to rosmarinic acid on the hemoglobin model (PDB: 5E83). Our focus was on the alpha cleft between two alpha chains of the hemoglobin model (PDB: 5E83). The results showed that most compounds had molecular weights above 500 daltons, and some exhibited acceptable hydrophobicity. Furthermore, their solubility in aqueous solutions was good. None of the compounds were able to cross the blood-brain barrier or have gastrointestinal absorption. However, they did have varying inhibitory effects on CYP2C9 cytochromes. The skin penetration rate was generally low.
Conclusion: Through our study, we identified three compounds (CID: 162739375, CID: 141386569, and CID: 24015539) with promising potential for further research. These compounds demonstrated high binding affinity to the hemoglobin model, favorable dissolution and digestive absorption rates, as well as suitable hydrophobicity, making them ideal candidates for continued laboratory investigation
Basic Medicine
Jaya B Puri; AbdulBasit M.A. Shaikh; Umesh Pravin Dhuldhaj
Abstract
In the present study, we have collected the leucocephala seeds which are seasonal and having good amount of oil content. The oil seeds can be used as the one of the adulterant for the coffee. The whole plant having importance for human being, it could be as the food or furniture. The immature seeds, ...
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In the present study, we have collected the leucocephala seeds which are seasonal and having good amount of oil content. The oil seeds can be used as the one of the adulterant for the coffee. The whole plant having importance for human being, it could be as the food or furniture. The immature seeds, pods and flower buds are used as the salad while the gum obtained from this plant can be used for the confectionary, pharmaceutics, and cosmetics industry. In some of the region, it is considered as the recalcitrant and attracts rodents. The plant is well-known in traditional medicine and seeds are the rich source of several necessary metabolites such as proteins, tannins, carotene, leucenol, and flavonol glycosides and having therapeutic potential like anti-inflammatory and antidiabetic activity. Hence, in our present investigation, we focus for the collection of seeds, and extraction of oil and its characterizations. The dried pods and seeds were collected from Waghi Village, near Nanded, Maharashtra. The obtained extract were further characterized for the confirmation of oil content by measuring lipid content, specific gravity, density, protein, and glucose content, and further analysis with thin layer chromatography. The obtained extract is also subjected to the anti-microbial activity.
Basic Medicine
Jaya B Puri; Abdulbasit Mustakahmad Shaikh; Umesh Pravin Dhuldhaj
Abstract
Since ancient times, Ajwain is one of the popular ayurvedic medicines in the treatment of indigestion, dyspepsia, and gastric disorders. Phytochemical constituents of Ajwain, are day by day getting more attention as medicine because of their efficiency and safer, and also comes with very less side effects. ...
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Since ancient times, Ajwain is one of the popular ayurvedic medicines in the treatment of indigestion, dyspepsia, and gastric disorders. Phytochemical constituents of Ajwain, are day by day getting more attention as medicine because of their efficiency and safer, and also comes with very less side effects. The natural compounds obtained from this ethno-botanical plants are received much more attention in last decades.The present investigation is focus on the phytochemical analysis of Ajwain seeds which is commonly used as one of the important ingredients of Indian mouth fresheners. The crude extract of Ajwain seeds were obtained through soxhletion in chloroform and water. The presence of secondary metabolites in the Ajwain seeds was done by qualitative and quantitative analysis. The qualitative analysis of crude aqueous extract of Ajwain seeds shown the presence of Cardiac glycosides, as one of the very important cardiac drug used in the heart treatments.
Basic Medicine
Parmiss Adyani Kalvanagh; Yousef Adyani Kalvanagh
Abstract
Introduction: This research investigates the relationship between A/T251 polymorphism of IL-8 gene (as a genetic marker) with cancer recurrence after lumpectomy and instead of PCR-RFLP method which most common and expensive method for detecting polymorphisms is the more economical technique of PCR Tetra ...
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Introduction: This research investigates the relationship between A/T251 polymorphism of IL-8 gene (as a genetic marker) with cancer recurrence after lumpectomy and instead of PCR-RFLP method which most common and expensive method for detecting polymorphisms is the more economical technique of PCR Tetra Arms.Methodology: After DNA extraction, a nano drop device was used to evaluate the quantity and quality of DNA and to know its concentration and degree of purity. 1 microliter of the extracted DNA was placed in the device and its optical absorption was read at different wavelengths. For the correct performance of the method and in order to determine the sequence and confirm the PCR product, several samples of dominant homozygotes, recessive homozygotes and heterozygotes were selected and purified by Cena clone kit.Results: Heterozygous individuals were identified with bp169 and bp228 long fragments along with bp439 control band. If mutant homozygous individuals with A allele showed bp 228 fragment and wild homozygous individuals with T allele showed bp 169 fragment on 2% electrophoresis gel.Conclusion: Several cytokines, including IL-8, play an important role in causing laminogenic inflammation in tissue and as a result, causing and aggravating cancers. These studies have studied the role of IL-8 gene polymorphisms and its relationship with types of cancer in different populations. Therefore, this study was conducted with the aim of investigating the 251 A/T polymorphism of the IL-8 gene in the population of Iranian women with breast cancer and the results of this study showed.
Basic Medicine
Ronak Rahimiyan
Abstract
Network methods are one of the most widely used groundwater modeling tools that have become widespread and popular in the last two decades. On the other hand, advances in the computing power of computers and their ease of access have led to the rapid development of numerical methods for solving groundwater ...
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Network methods are one of the most widely used groundwater modeling tools that have become widespread and popular in the last two decades. On the other hand, advances in the computing power of computers and their ease of access have led to the rapid development of numerical methods for solving groundwater problems. In this study, with a new approach to network methods, these methods are introduced as a numerical model to simulate the movement of groundwater. The first step in estimating groundwater behavior is to obtain a mathematical model. According to Darcy's law and the establishment of mass conservation, it can be shown that the equation governing groundwater in stable conditions is the Laplace equation. Therefore, by obtaining the physical properties of the desired aquifer using the experiment and understanding the boundary conditions governing that aquifer, a complete mathematical model governing the desired problem can be obtained. Unfortunately, since the physical properties of the problems in nature are not homogeneous and the boundaries of the problems under study are geometrically irregular, it is impossible to solve these problems analytically. To do so, researchers have been using numerical and laboratory methods for years. All numerical methods for solving the Laplace equation first decompose the scope of the problem in the sense that they either divide it into multiple nodes or into multiple elements and then use mathematical methods. In this article, a new approach is to find algebraic relations between those different nodes or elements. In other words, after parsing the scope of the problem, they turn the Laplace equation into a system of linear equations.
