Basic Medicine
Parmiss Adyani Kalvanagh; Yousef Adyani Kalvanagh
Abstract
Introduction: This research investigates the relationship between A/T251 polymorphism of IL-8 gene (as a genetic marker) with cancer recurrence after lumpectomy and instead of PCR-RFLP method which most common and expensive method for detecting polymorphisms is the more economical technique of PCR Tetra ...
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Introduction: This research investigates the relationship between A/T251 polymorphism of IL-8 gene (as a genetic marker) with cancer recurrence after lumpectomy and instead of PCR-RFLP method which most common and expensive method for detecting polymorphisms is the more economical technique of PCR Tetra Arms.Methodology: After DNA extraction, a nano drop device was used to evaluate the quantity and quality of DNA and to know its concentration and degree of purity. 1 microliter of the extracted DNA was placed in the device and its optical absorption was read at different wavelengths. For the correct performance of the method and in order to determine the sequence and confirm the PCR product, several samples of dominant homozygotes, recessive homozygotes and heterozygotes were selected and purified by Cena clone kit.Results: Heterozygous individuals were identified with bp169 and bp228 long fragments along with bp439 control band. If mutant homozygous individuals with A allele showed bp 228 fragment and wild homozygous individuals with T allele showed bp 169 fragment on 2% electrophoresis gel.Conclusion: Several cytokines, including IL-8, play an important role in causing laminogenic inflammation in tissue and as a result, causing and aggravating cancers. These studies have studied the role of IL-8 gene polymorphisms and its relationship with types of cancer in different populations. Therefore, this study was conducted with the aim of investigating the 251 A/T polymorphism of the IL-8 gene in the population of Iranian women with breast cancer and the results of this study showed.
Basic Medicine
Saeed Saedi; Atefeh Saedi; Mohammad Mehdi Ghaemi; Maryam Milani Fard
Abstract
Breast cancer is defined as changes in the uncontrolled growth of cells in breast tissue that occur abnormally in the mammary glands (lobules) or in the ducts that connect the lobules to the nipple. The present study is an epidemiological study of breast cancer in Iran. The browsing documents searched ...
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Breast cancer is defined as changes in the uncontrolled growth of cells in breast tissue that occur abnormally in the mammary glands (lobules) or in the ducts that connect the lobules to the nipple. The present study is an epidemiological study of breast cancer in Iran. The browsing documents searched in the Google Scholar, Sid and Mag Iran databases were analyzed using the keywords epidemic, cancer, breast. Based on the results obtained in this study, the incidence and death rate and burden of breast cancer in Iran has risen significantly, compared with previous years. Because of the vital role of women in the family, economy, health, development and other areas, early detection of breast cancer and its prevention according to its high prevalence around the world, as one of the best approaches to control this disease, is important.
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