Basic Medicine
Parmiss Adyani Kalvanagh; Yousef Adyani Kalvanagh
Abstract
Introduction: This research investigates the relationship between A/T251 polymorphism of IL-8 gene (as a genetic marker) with cancer recurrence after lumpectomy and instead of PCR-RFLP method which most common and expensive method for detecting polymorphisms is the more economical technique of PCR Tetra ...
Read More
Introduction: This research investigates the relationship between A/T251 polymorphism of IL-8 gene (as a genetic marker) with cancer recurrence after lumpectomy and instead of PCR-RFLP method which most common and expensive method for detecting polymorphisms is the more economical technique of PCR Tetra Arms.Methodology: After DNA extraction, a nano drop device was used to evaluate the quantity and quality of DNA and to know its concentration and degree of purity. 1 microliter of the extracted DNA was placed in the device and its optical absorption was read at different wavelengths. For the correct performance of the method and in order to determine the sequence and confirm the PCR product, several samples of dominant homozygotes, recessive homozygotes and heterozygotes were selected and purified by Cena clone kit.Results: Heterozygous individuals were identified with bp169 and bp228 long fragments along with bp439 control band. If mutant homozygous individuals with A allele showed bp 228 fragment and wild homozygous individuals with T allele showed bp 169 fragment on 2% electrophoresis gel.Conclusion: Several cytokines, including IL-8, play an important role in causing laminogenic inflammation in tissue and as a result, causing and aggravating cancers. These studies have studied the role of IL-8 gene polymorphisms and its relationship with types of cancer in different populations. Therefore, this study was conducted with the aim of investigating the 251 A/T polymorphism of the IL-8 gene in the population of Iranian women with breast cancer and the results of this study showed.
Basic Medicine
Parmiss Adyani Kalvanagh; Yousef Adyani Kalvanagh
Abstract
Introduction: According to researchers' research on the relationship between breast cancer and diabetes in the presence of other genes such as INSR, the aim of this study was comparing exons 2 and 3 of the DIRAS3 gene in mastectomies and lumpectomies women.Procedure: DNA extraction was done using a kit ...
Read More
Introduction: According to researchers' research on the relationship between breast cancer and diabetes in the presence of other genes such as INSR, the aim of this study was comparing exons 2 and 3 of the DIRAS3 gene in mastectomies and lumpectomies women.Procedure: DNA extraction was done using a kit (PZP Molecular IVD Company) and stored in a micro tube at -20C. The said kit contained four different buffers named Wash buffer (W), Binding buffer (B), Elution buffer (E) and Removal buffer (R) along with proteinase k powder.Results: The polymorphism found in intron 2 with rs6682360 at position c.332-3 T>C or g.831 T>C of a cancer patient sample, that is, 3 nucleotides upstream of the 332nd nucleotide in intron 2 or the 831st nucleotide in intron 2 which is T open to C open. Since the number of measured samples was small, no significant relationship was found between the development of breast cancer and diabetes and the presence of this polymorphism.Conclusion: The results of this research showed that among the available polymorphisms, only the 5'UTR c.331 G>A is related to breast cancer in mastectomies and lumpectomies women.
